GAPDH Polyclonal Antibody

Product Specifications

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Abbreviation

GAPDH

UniProt

Q5XJ10

Host

Rabbit

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein

Target

Aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Neuroscience; Isotype/Loading Controls; Signal Transduction; Cancer; Metabolism

Purification

Antigen Affinity Purification

Concentration

1 mg/mL

Dilution

WB 1:3000-1:6000; IHC 1:300-1:500

Buffer

PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

35 kDa

Observed Molecular Weight

35 kDa

Citation 01

https://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btm2.10275

Citation 02

https://papers.ssrn.com/sol3/papers.cfm?abstract_id=3919938

Citation 03

https://pubmed.ncbi.nlm.nih.gov/35860242/

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332222006783

Citation 05

https://www.mdpi.com/1579044

Citation 06

https://www.sciencedirect.com/science/article/pii/S0223523422003117

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019468/

Citation 08

https://www.sciencedirect.com/science/article/pii/S1567576922004763

Citation 09

https://www.sciencedirect.com/science/article/pii/S1567576923000449

Citation 10

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053591/

Citation 11

https://iovs.arvojournals.org/article.aspx?articleid=2783840

Citation 12

https://www.sciencedirect.com/science/article/pii/S1567576921000928

Isotype

IgG

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

abx114237 100 µL

Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1) Antibody

Ask

View Details

FAB179X 0.1 mL

Mouse Monoclonal CCR9 Antibody (112509) [Alexa Fluor 532]

Ask

View Details

CTK-377 96 Tests

Human Complement C6 ELISA Kit

Ask

View Details

RG-067 1x 10^6

PK-15-Cas9 Cell Line

Ask

View Details

RR211676L3 10 µg

Sh2d1a (NM_001109313) Rat Tagged Lenti ORF Clone

Ask

View Details