ERK 1/2 Polyclonal Antibody
Product Specifications
Background
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2) . Phosphorylates SPZ1 (By similarity) . Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Abbreviation
ERK 1/2
UniProt
P27361, P28482
Host
Rabbit
Reactivity
Human; Mouse; Rat
Immunogen
Synthesized peptide derived from human ERK 1/2 around the non-phosphorylation site of Thr202.
Target
MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB; IF
Field of Research
Cancer; Signal Transduction; Neuroscience
Purification
Affinity purification
Concentration
1 mg/mL
Dilution
WB 1:500-1:2000; IF 1:100-1:300
Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
43kDa
Observed Molecular Weight
44+42kDa
Isotype
IgG
Available Sizes
Frequently Asked Questions
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