AMPK alpha1/2 Polyclonal Antibody
Product Specifications
Background
AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.
Abbreviation
AMPKα1/2
UniProt
Q13131, P54646
Host
Rabbit
Reactivity
Human; Mouse; Rat; Monkey
Immunogen
Synthesized peptide derived from human AMPKα1/2 around the non-phosphorylation site of Thr183/172.
Target
PRKAA1; AMPK1;5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB; IHC-p
Field of Research
Cancer; Cardiovascular; Metabolism; Neuroscience; Signal Transduction
Purification
Affinity purification
Concentration
1 mg/mL
Dilution
WB 1:500-1:2000; IHC 1:100-1:300
Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
62kDa
Observed Molecular Weight
63kDa
Isotype
IgG
Available Sizes
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