Alpha Tubulin Monoclonal Antibody

Product Specifications

Background

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Abbreviation

Α-tubulin

UniProt

Q71U36, P68363

Host

Mouse

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant Protein

Target

TUBA1A; TUBA3; Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA1B; Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain

Clonality

Monoclonal

Clone

Clone:5M5

Conjugation

Unconjugated

Applications

WB; IHC-p; IF; IP

Field of Research

Cancer; Signal Transduction

Purification

Protein A purification

Concentration

1 mg/mL

Dilution

WB 1:500-10000; IHC 1:50-300; IF 1:50-1:200; IP 1:100-1:300

Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

50kDa

Observed Molecular Weight

52kDa

Citation 01

https://www.sciencedirect.com/science/article/pii/S0269749122003207

Citation 02

https://www.frontiersin.org/articles/10.3389/fimmu.2021.636614/full

Citation 03

https://www.mdpi.com/2076-3921/11/8/1611

Citation 04

https://www.sciencedirect.com/science/article/pii/S2212877821001848

Citation 05

https://www.mdpi.com/1975256

Citation 06

https://www.mdpi.com/1315988

Citation 07

https://www.mdpi.com/1422-0067/23/15/8464

Citation 08

https://www.mdpi.com/1841724

Citation 09

https://www.mdpi.com/1660-3397/20/10/595

Citation 10

https://www.mdpi.com/1422-0067/22/15/8117

Citation 11

https://www.mdpi.com/1422-0067/23/9/5128

Citation 12

https://www.mdpi.com/1422-0067/22/18/10120

Isotype

IgG

Available Sizes

Curated Selection

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