ADO Polyclonal Antibody
Product Specifications
Background
Human thiol dioxygenases include cysteine dioxygenase (CDO, MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO, EC 1.13.11.19) . CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.
Abbreviation
ADO
UniProt
Q96SZ5
Host
Rabbit
Reactivity
Human; Mouse
Immunogen
Synthetic peptide of human ADO
Target
2 aminoethanethiol dioxygenase;2-aminoethanethiol dioxygenase; ADO 2 aminoethanethiol (cysteamine) dioxygenase; ADO; AEDO; C10orf22; Chromosome 10 open reasing frame 22; cysteamine (2-aminoethanethiol) dioxygenase (ADO) ; Cysteamine dioxygenase; DKFZp564C046; FLJ14547
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB; IHC
Field of Research
Metabolism; Signal Transduction
Purification
Affinity purification
Concentration
0.3 mg/mL
Dilution
WB 1:1000-1:5000; IHC 1:25-1:100
Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Product Datasheet
https://789.bio/ea/8thhw5
Product MSDS
https://789.bio/eb/jzP0y5
Calculated Molecular Weight
30kDa
Isotype
IgG
Available Sizes
Curated Selection
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