RBFOX3 Polyclonal Antibody
Product Specifications
Background
Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.
Abbreviation
RBFOX3
UniProt
A6NFN3
Host
Rabbit
Reactivity
Human ; Mouse
Immunogen
Synthetic peptide of human RBFOX3
Target
FLJ56884; FLJ58356; Fox-1 homolog C; fox1 homolog C; Fox3; FOX3NeuN; hexaribonucleotide binding protein 3; HRNBP3; NEUN; neuronal nuclei; Rbfox3; RFOX3; RNA binding protein fox-1 homolog 3; RNA binding protein; fox 1 homolog (C. elegans) 3
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB; IHC
Field of Research
Cancer; Epigenetics and Nuclear Signaling; Neuroscience; Tags and Cell Markers
Purification
Affinity purification
Concentration
0.1 mg/mL
Dilution
WB 1:200-1:1000; IHC 1:50-200
Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Product Datasheet
https://789.bio/ea/ishdkg
Product MSDS
https://789.bio/eb/yfz5yH
Calculated Molecular Weight
34kDa
Observed Molecular Weight
Refer to figures
Isotype
IgG
Available Sizes
Curated Selection
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