AHA1 Protein

Human Recombinant AHA1 Protein

Product Specifications

Product Name Alternative

AHSA1, AHA1, HSPC322, p38, C14orf3, LOC10598, Activator of Hsp90 ATPase activity 1, Activator of 90 kDa heat shock protein ATPase homolog 1

UniProt

O95433

Reactivity

Human

Tag

His tag

Source

Recombinant

Origin

Human

Field of Research

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Concentration

Lot/batch specific. See included datasheet.

Purity

>90%

Molecular Weight

~38 kDa

Storage Conditions

-20°C

Notes

For research use only.

Applications Notes

This product has been certified >90% pure using SDS PAGE analysis. 2uM orb1714238 generated a 9-fold ATPase activation of 2uM HSP90 (His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)

Tested Applications

FA, SDS-PAGE, WB

Preservative

20mM HEPES buffer pH 7.2, 80mM NaCl, 10% glycerol

AA Sequence

SHMAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTDKLKTLFLAVQVQNEEGKCEVTEVSKLDGEASINNRKGKLIFFYEWSVKLNWTGTSKSGVQYKGHVEIPNLSDENSVDEVEISVSLAKDEPDTNLVALMKEEGVKLLREAMGIYISTLKTEFTQGMILPTMNGESVDPVGQPALKTEERKAKPAPSKTQARPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPATLEADRGGKFHMVDGNVSGEFTDLVPEKHIVMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF