Purified Anti-Human CD22 Antibody[HIB22]

Product Specifications

Background

CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM. It is a member of the immunoglobulin superfamily (sialoadhesion subgroup) . CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.

Abbreviation

CD22

Gene ID

933

UniProt

P20273

Host

Mouse

Reactivity

Human

Target

Siglec2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; CD22; Cd22; Lyb-8; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14

Clonality

Monoclonal

Clone

HIB22

Conjugation

Unconjugated

Applications

FCM

Field of Research

Immunology

Concentration

≥ 1 mg/mL

Buffer

Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

Recommended Usage

Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.