Purified Anti-Mouse IL-6 Antibody[MP5-20F3]
Product Specifications
Background
IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.
Abbreviation
IL-6
UniProt
P08505
Host
Rat
Reactivity
Mouse
Target
B-cell hybridoma growth factor; IL-6; Interleukin HP-1; Interleukin-6
Clonality
Monoclonal
Clone
MP5-20F3
Conjugation
Unconjugated
Applications
ICFCM
Field of Research
Immunology; Innate Immunity
Concentration
≥ 1 mg/mL
Buffer
Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.
Shipping Conditions
Ice bag
Storage Conditions
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.
Citation 01
https://www.nature.com/articles/s41419-023-05890-1
Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 0.5 – 2.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-6 recombinant protein ranging from 500 to 8 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Isotype
Rat IgG1, κ
Available Sizes
Frequently Asked Questions
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