AF/LE Purified Anti-Mouse IL-6 Antibody[MP5-20F3]
Product Specifications
Background
IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.
Abbreviation
IL-6
UniProt
P08505
Host
Rat
Reactivity
Mouse
Target
B-cell hybridoma growth factor; IL-6; Interleukin HP-1; Interleukin-6
Clonality
Monoclonal
Clone
MP5-20F3
Conjugation
None (AF/LE)
Applications
ICFCM; Neut
Field of Research
Immunology; Innate Immunity
Concentration
≥ 1 mg/mL
Buffer
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
Shipping Conditions
Ice bag
Storage Conditions
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 0.5 – 2.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-6 recombinant protein ranging from 500 to 8 pg/mL are recommended for each ELISA plate. For ELISPOT applications, a concentration range of 4.0 - 8.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Isotype
Rat IgG1, κ
Available Sizes
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