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AF/LE Purified Anti-Mouse IL-4 Antibody[11B11]

Product Specifications

Background

IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development.

Abbreviation

IL-4

Gene ID

16189

UniProt

P07750

Host

Rat

Reactivity

Mouse

Target

B-cell IgG differentiation factor; B-cell growth factor 1; BSF-1; IGG1 induction factor; IL-4; Interleukin-4

Clonality

Monoclonal

Clone

11B11

Conjugation

None (AF/LE)

Applications

ICFCM; Neut; Stim

Field of Research

Immunology

Concentration

≥ 1 mg/mL

Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Shipping Conditions

Ice bag

Storage Conditions

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Datasheet

https://789.bio/ea/ufvdap

Product MSDS

https://789.bio/eb/5ur5yL

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISPOT applications, a concentration range of 2-6 μg/ml is recommended. For ELISA capture applications, a concentration range of 0.5-2.0 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-4 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype

Rat IgG1, κ

Available Sizes

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