AF/LE Purified Anti-Mouse IL-4 Antibody[11B11]
Product Specifications
Background
IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development.
Abbreviation
IL-4
Gene ID
16189
UniProt
P07750
Host
Rat
Reactivity
Mouse
Target
B-cell IgG differentiation factor; B-cell growth factor 1; BSF-1; IGG1 induction factor; IL-4; Interleukin-4
Clonality
Monoclonal
Clone
11B11
Conjugation
None (AF/LE)
Applications
ICFCM; Neut; Stim
Field of Research
Immunology
Concentration
≥ 1 mg/mL
Buffer
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
Shipping Conditions
Ice bag
Storage Conditions
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISPOT applications, a concentration range of 2-6 μg/ml is recommended. For ELISA capture applications, a concentration range of 0.5-2.0 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-4 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Isotype
Rat IgG1, κ
Available Sizes
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