Glutathione Peroxidase 4 (GPX4) Activity Assay Kit
Product Specifications
Target
GPX4
Type
Colorimetric method
Field of Research
Ferroptosis
Assay Principle
Glutathione peroxidase 4 (GPX4) is a kind of glutathione peroxidase containing selenocysteine, which can reduce phospholipid hydroperoxide to phosphatidyl alcohol, thus protecting the cell membrane from oxidative damage. GPX4 catalyzes the substrate, and the product consumes the reducing agent with the addition of enzyme reagents.The reducing agent has a maximum absorbance at 340 nm, and the GP4-specific enzyme activity can be calculated by measuring non-specific enzyme activity and total enzyme activity by adding GPX-4 inhibitor to the system.
Sample Type
Animal tissue; cell
Detection Range
3.22-44.69U/L
Sensitivity
3.22U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
0.4-3.4%
Intra CV
1.2-3.1%
Detection Instrument
Microplate reader
Available Sizes
Documents
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog