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Glutathione Peroxidase 4 (GPX4) Activity Assay Kit

Product Specifications

Target

GPX4

Type

Colorimetric method

Field of Research

Ferroptosis

Assay Principle

Glutathione peroxidase 4 (GPX4) is a kind of glutathione peroxidase containing selenocysteine, which can reduce phospholipid hydroperoxide to phosphatidyl alcohol, thus protecting the cell membrane from oxidative damage. GPX4 catalyzes the substrate, and the product consumes the reducing agent with the addition of enzyme reagents.The reducing agent has a maximum absorbance at 340 nm, and the GP4-specific enzyme activity can be calculated by measuring non-specific enzyme activity and total enzyme activity by adding GPX-4 inhibitor to the system.

Sample Type

Animal tissue; cell

Detection Range

3.22-44.69U/L

Sensitivity

3.22U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

0.4-3.4%

Intra CV

1.2-3.1%

Detection Instrument

Microplate reader

Available Sizes

Frequently Asked Questions

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