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Pepsin Activity Assay Kit

Product Specifications

Type

Colorimetric method

Field of Research

Amino Acids and Proteins

Assay Principle

Pepsin can catalyze substrate hydrolysis, and the hydrolysate reacts with the chromogenic agent under alkaline conditions to form a blue substance. The blue substance has a maximum absorption peak at 660 nm, and the color depth is proportional to the activity of pepsin. The enzyme activity can be calculated by OD value

Sample Type

Gastric; intestinal tissue

Detection Range

0.63-94.98 U/mL

Sensitivity

0.63 U/mL

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

4.8-5.6%

Intra CV

1.3-2.2%

Detection Instrument

Microplate reader

Frequently Asked Questions

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