Pepsin Activity Assay Kit
Product Specifications
Type
Colorimetric method
Field of Research
Amino Acids and Proteins
Assay Principle
Pepsin can catalyze substrate hydrolysis, and the hydrolysate reacts with the chromogenic agent under alkaline conditions to form a blue substance. The blue substance has a maximum absorption peak at 660 nm, and the color depth is proportional to the activity of pepsin. The enzyme activity can be calculated by OD value
Sample Type
Gastric; intestinal tissue
Detection Range
0.63-94.98 U/mL
Sensitivity
0.63 U/mL
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
4.8-5.6%
Intra CV
1.3-2.2%
Detection Instrument
Microplate reader
Documents
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