Glutaminase (GLS) Activity Assay Kit
Product Specifications
Target
GLS
Type
Colorimetric method
Field of Research
Amino Acids and Proteins
Assay Principle
Glutamine is decomposed to produce glutamic acid under the action of glutaminase. Glutamic acid is further transformed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of glutaminase can be calculated by measuring the change of absorbance value at 450 nm.
Assay Performance Time
80min
Sample Type
Serum (plasma) ; animal; plant tissue
Detection Range
0.003-18.0 U/L
Sensitivity
0.003 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
8.3%
Intra CV
5%
Detection Instrument
Microplate reader
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