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Phosphofructokinase (PFK) Activity Assay Kit

Product Specifications

Target

PFK

Type

Colorimetric method

Field of Research

Glycolysis and Lipid Metabolism

Assay Principle

Phosphofructokinase (also known as 6-phosphofructokinase; PFK) is a class of kinases that act on fructose-6-phosphate. Phosphofructokinase catalyzes fructose-6-phosphate and ATP to produce fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. The activity of PFK can be reflected by the determination of NADH decline rate at 340 nm.

Assay Performance Time

15 min

Sample Type

Serum; plasma; tissue; cell sample

Detection Range

0.27-32.29 U/L

Sensitivity

0.27 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

8%

Intra CV

3%

Detection Instrument

Microplate reader

Available Sizes

Frequently Asked Questions

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