Phosphofructokinase (PFK) Activity Assay Kit
Product Specifications
Target
PFK
Type
Colorimetric method
Field of Research
Glycolysis and Lipid Metabolism
Assay Principle
Phosphofructokinase (also known as 6-phosphofructokinase; PFK) is a class of kinases that act on fructose-6-phosphate. Phosphofructokinase catalyzes fructose-6-phosphate and ATP to produce fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. The activity of PFK can be reflected by the determination of NADH decline rate at 340 nm.
Assay Performance Time
15 min
Sample Type
Serum; plasma; tissue; cell sample
Detection Range
0.27-32.29 U/L
Sensitivity
0.27 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
8%
Intra CV
3%
Detection Instrument
Microplate reader
Available Sizes
Documents
Frequently Asked Questions
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