Pyruvate Kinase (PK) Activity Assay Kit
Product Specifications
Target
PK
Type
Colorimetric method
Field of Research
Glycolysis and Lipid Metabolism; Tricarboxylic Acid (TCA) Cycle
Assay Principle
In the presence of adenosine diphosphate (ADP), pyruvate kinase (PK) catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, Lactate dehydrogenase catalyzes the reaction of pyruvic acid with NADH to produce lactic acid and NAD+. The activity of pyruvate kinase can be calculated by detection the absorbance changes at 340 nm.
Assay Performance Time
10 min
Sample Type
Serum; plasma; tissue; cell
Detection Range
0.08–6.79 U/L
Sensitivity
0.08 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
7%
Intra CV
5%
Detection Instrument
Microplate reader
Available Sizes
Documents
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC940031-100 1x 100 µL
Mucin 5AC (MUC5AC) (45M1), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC040031-100 1x 100 µL
Mucin 5AC (MUC5AC) (45M1), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC470460-100 1x 100 µL
CD44 Standard (156-3C11), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC742091-500 1x 500 µL
P53 Tumor Suppressor Protein (rTP53/1739), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC740268-100 1x 100 µL
CD3e (T-Cell Marker) (OKT3), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details