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Hexokinase (HK) Activity Assay Kit

Product Specifications

Target

HK

Type

Colorimetric method

Field of Research

Glycolysis and Lipid Metabolism

Assay Principle

HK converts glucose to glucose 6-phosphate, which is catalyzed by the enzyme. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of HK can be calculated by measuring the change of absorbance value at 450 nm.

Assay Performance Time

30 min

Sample Type

Animal tissue; cell

Detection Range

5.93-40.11 U/L

Sensitivity

5.93 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

9.1%

Intra CV

3.3%

Detection Instrument

Microplate reader

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