Hexokinase (HK) Activity Assay Kit
Product Specifications
Target
HK
Type
Colorimetric method
Field of Research
Glycolysis and Lipid Metabolism
Assay Principle
HK converts glucose to glucose 6-phosphate, which is catalyzed by the enzyme. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of HK can be calculated by measuring the change of absorbance value at 450 nm.
Assay Performance Time
30 min
Sample Type
Animal tissue; cell
Detection Range
5.93-40.11 U/L
Sensitivity
5.93 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
9.1%
Intra CV
3.3%
Detection Instrument
Microplate reader
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