Pyruvate Decarboxylase (PDC) Activity Assay Kit
Product Specifications
Target
PDC
Type
Colorimetric method
Field of Research
Glycolysis and Lipid Metabolism
Assay Principle
Pyruvate decarboxylase (PDC) mainly exists in yeast and is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylate to acetaldehyde, which reacts under the action of ethanol dehydrogenase (ADH), and catalyzes NADH to convert into NAD+. NADH has a characteristic absorption peak at 340 nm. The PDC activity can be calculated by measuring the OD value at 340 nm.
Assay Performance Time
15 min
Sample Type
Serum; plasma; tissue; cell samples
Detection Range
0.67-27.73 U/L
Sensitivity
0.67 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
3.1%
Intra CV
2.8%
Detection Instrument
Microplate reader
Available Sizes
Documents
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