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Ascorbate Peroxidase (APX) Activity Assay Kit

Product Specifications

Target

APX

Type

Colorimetric method

Field of Research

Plant Physiology Series

Assay Principle

Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA) . The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290.

Assay Performance Time

60 min

Sample Type

Plant tissue

Detection Range

0.071-47 U/g tissue

Sensitivity

0.071 U/g tissue

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://www.sciencedirect.com/science/article/pii/S1674205221001659

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12504

Citation 03

https://www.frontiersin.org/articles/10.3389/fpls.2022.941388/full

Citation 04

https://www.sciencedirect.com/science/article/pii/S0981942820300784

Citation 05

https://www.mdpi.com/2223-7747/10/3/541

Citation 06

https://peerj.com/articles/11888/

Citation 07

https://journals.ashs.org/hortsci/view/journals/hortsci/56/9/article-p1066.xml

Inter CV

6.4%

Intra CV

4.8%

Detection Instrument

Spectrophotometer

Available Sizes

Frequently Asked Questions

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