Ascorbate Peroxidase (APX) Activity Assay Kit
Product Specifications
Target
APX
Type
Colorimetric method
Field of Research
Plant Physiology Series
Assay Principle
Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA) . The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290.
Assay Performance Time
60 min
Sample Type
Plant tissue
Detection Range
0.071-47 U/g tissue
Sensitivity
0.071 U/g tissue
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2~8°C for 12 months with shading light.
Citation 01
https://www.sciencedirect.com/science/article/pii/S1674205221001659
Citation 02
https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12504
Citation 03
https://www.frontiersin.org/articles/10.3389/fpls.2022.941388/full
Citation 04
https://www.sciencedirect.com/science/article/pii/S0981942820300784
Citation 05
https://www.mdpi.com/2223-7747/10/3/541
Citation 06
https://peerj.com/articles/11888/
Citation 07
https://journals.ashs.org/hortsci/view/journals/hortsci/56/9/article-p1066.xml
Inter CV
6.4%
Intra CV
4.8%
Detection Instrument
Spectrophotometer
Available Sizes
Documents
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