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Urea (BUN) Colorimetric Assay Kit (Diacetyl Oxime Method)

Product Specifications

Target

BUN

Type

Colorimetric method

Field of Research

Liver and Renal Function

Assay Principle

In strong acidic and heating condition, urea can react with diacetyl to form red diazine compound. The depth of color is proportional to the content of urea. Because the instability of the diacetyl, the diacetyl oxime usually react with the strong acid firstly in the reaction system to generate diacetyl, then react with urea to generate the red diazine compound. The reaction equation is as follows:

Assay Performance Time

60 min

Sample Type

Serum; plasma; saliva; urine

Detection Range

0.12-15 mmol/L

Sensitivity

0.12 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://www.sciencedirect.com/science/article/pii/S1742706121007108

Citation 02

https://www.hindawi.com/journals/omcl/2022/1763922/

Citation 03

https://www.mdpi.com/2124124

Citation 04

https://www.mdpi.com/article/10.3390/ijms232315400

Citation 05

https://europepmc.org/article/med/35392552?javascript_support=no

Citation 06

https://www.sciencedirect.com/science/article/pii/S0753332220308775

Citation 07

https://www.tandfonline.com/doi/abs/10.1080/09603123.2022.2105824

Citation 08

https://www.frontiersin.org/articles/10.3389/fphys.2023.1083725/full?utm_source=dlvr.it&utm_medium=twitter

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525417/

Citation 10

https://academic.oup.com/jpp/advance-article-abstract/doi/10.1093/jpp/rgab078/6299670

Citation 11

https://www.mdpi.com/1433254

Inter CV

9.9%

Intra CV

4.9%

Detection Instrument

Spectrophotometer

Available Sizes

Curated Selection

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