Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)

Product Specifications

Target

Type

Colorimetric method

Field of Research

Metabolic Diseases; Glycolysis and Lipid Metabolism

Assay Principle

Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK) . Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO) . In the presence of 4-aminoantipyrine and phenol, hydrogen peroxide is catalyzed by peroxidase (POD) to produce quinones which is proportional to the content of TG.

Assay Performance Time

30 min

Sample Type

Serum; plasma; tissue

Detection Range

0.14-10 mmol/L

Sensitivity

0.14 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://www.nature.com/articles/s41467-022-35481-1

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202204909

Citation 03

https://www.sciencedirect.com/science/article/pii/S0142961222005427

Citation 04

https://www.sciencedirect.com/science/article/pii/S0048969722056844

Citation 05

https://papers.ssrn.com/sol3/papers.cfm?abstract_id=3876697

Citation 06

https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-021-01225-8

Citation 07

https://www.sciencedirect.com/science/article/pii/S1043661819308473

Citation 08

https://www.sciencedirect.com/science/article/pii/S004896972105405X

Citation 09

https://www.sciencedirect.com/science/article/pii/S0223523421002373

Citation 10

https://pubs.rsc.org/en/content/articlelanding/2023/fo/d2fo03509j

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279576/

Citation 12

https://www.frontiersin.org/articles/10.3389/fphar.2021.689111/full

Inter CV

9.2%

Intra CV

4.1%

Detection Instrument

Microplate reader

Available Sizes

Documents

Product Datasheet

Click to open

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