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Aspartate Aminotransferase (AST/GOT) Activity Assay Kit

Product Specifications

Target

AST; GOT; Glutamic-oxalacetic transaminase

Type

Colorimetric method

Field of Research

Liver and Renal Function

Assay Principle

AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.

Assay Performance Time

80 min

Sample Type

Serum; plasma; animal tissue; cells; cell culture supernatant

Detection Range

1.10-72.30 IU/L

Sensitivity

1.10 IU/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202204909

Citation 02

https://www.nature.com/articles/s41467-022-29722-6

Citation 03

https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep.29573

Citation 04

https://www.sciencedirect.com/science/article/pii/S2162253122002165

Citation 05

https://www.sciencedirect.com/science/article/pii/S2211124722011457

Citation 06

https://pubs.acs.org/doi/abs/10.1021/acsami.1c12235

Citation 07

https://www.sciencedirect.com/science/article/pii/S0304389420328673

Citation 08

https://pubs.acs.org/doi/abs/10.1021/acs.est.2c00516

Citation 09

https://www.sciencedirect.com/science/article/pii/S1742706120307509

Citation 10

https://www.sciencedirect.com/science/article/pii/S1742706121007108

Citation 11

https://www.sciencedirect.com/science/article/pii/S2352345X22001928

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13332

Inter CV

6.8%

Intra CV

5.3%

Detection Instrument

Microplate reader

Frequently Asked Questions

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