Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

Product Specifications

Target

ALT; GPT; Glutamic-pyruvic transaminase

Type

Colorimetric method

Field of Research

Liver and Renal Function

Assay Principle

ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37°C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

Assay Performance Time

100 min

Sample Type

Serum; plasma; cells; animal tissue

Detection Range

1.26-72.3 IU/L

Sensitivity

1.26 IU/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009960

Citation 02

https://www.mdpi.com/2073-4409/10/7/1622

Citation 03

https://www.sciencedirect.com/science/article/pii/S0378874122003452

Citation 04

https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-022-03536-x

Citation 05

https://peerj.com/articles/13082/

Citation 06

https://www.hindawi.com/journals/jnm/2021/7512957/

Citation 07

https://journals.sagepub.com/doi/abs/10.1177/0748233719893203

Citation 08

https://www.sciencedirect.com/science/article/pii/S2214750017301051

Inter CV

8.2%

Intra CV

4.3%

Detection Instrument

Spectrophotometer

Available Sizes

Documents

Product Datasheet

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