Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

Product Specifications

Target

ALT; GPT; Glutamic-pyruvic transaminase

Type

Colorimetric method

Field of Research

Liver and Renal Function

Assay Principle

ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37°C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

Assay Performance Time

80 min

Sample Type

Serum; plasma; animal tissue; cells; cell culture supernatant

Detection Range

0.75-72.30 IU/L

Sensitivity

0.75 IU/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202204909

Citation 02

https://www.nature.com/articles/s41467-022-35481-1

Citation 03

https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep.29573

Citation 04

https://www.sciencedirect.com/science/article/pii/S2162253122002165

Citation 05

https://www.sciencedirect.com/science/article/pii/S2211124722011457

Citation 06

https://pubs.acs.org/doi/abs/10.1021/acsami.1c12235

Citation 07

https://pubs.acs.org/doi/abs/10.1021/acs.est.2c00516

Citation 08

https://www.sciencedirect.com/science/article/pii/S0304389420328673

Citation 09

https://www.sciencedirect.com/science/article/pii/S2352345X22001928

Citation 10

https://www.sciencedirect.com/science/article/pii/S1742706121007108

Citation 11

https://www.sciencedirect.com/science/article/pii/S1043661819308473

Citation 12

https://www.sciencedirect.com/science/article/pii/S0048969722030819

Inter CV

Intra CV

5.3%

Detection Instrument

Microplate reader

Available Sizes

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