Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Enzyme Method)

Product Specifications

Target

T-AOC

Type

Colorimetric method

Field of Research

Oxidative stress; Plant Physiology Series

Assay Principle

The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 414 nm or 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.

Assay Performance Time

20 min

Sample Type

Serum; plasma; urine; saliva; tissue; cells

Detection Range

0.047-1.50 mmol/L

Sensitivity

0.047 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Citation 01

https://www.mdpi.com/article/10.3390/foods12040767

Citation 02

https://www.mdpi.com/1332228

Citation 03

https://static.frontiersin.org/articles/10.3389/fphar.2022.886514/full

Citation 04

https://pubmed.ncbi.nlm.nih.gov/34776947/

Citation 05

https://www.nature.com/articles/s41598-021-91256-6

Citation 06

https://www.sciencedirect.com/science/article/pii/S0753332220308775

Citation 07

https://www.dovepress.com/the-preventive-and-therapeutic-effects-of-ajwa-date-fruit-extract-agai-peer-reviewed-fulltext-article-DDDT

Citation 08

https://www.sciencedirect.com/science/article/pii/S037887412100893X

Citation 09

https://onlinelibrary.wiley.com/doi/abs/10.1111/aji.13589

Citation 10

https://www.tandfonline.com/doi/abs/10.1080/09205063.2022.2099668

Citation 11

https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.12010

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894603/

Inter CV

4.1%

Intra CV

2.2%

Detection Instrument

Microplate reader

Available Sizes

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