Creatinine (Cr) Colorimetric Assay Kit (Sarcosine Oxidase Method)

Product Specifications

Target

Type

Colorimetric method

Field of Research

Liver and Renal Function

Assay Principle

Creatinine (Cr) can be catalyzed by creatinase and generates creatine. Creatine can be hydrolyzed into sarcosine and urea by creatinase. The sarcosine can be catalyzed by sarcosine oxidase and form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4- (6-Tri-iodine-3- hydroxybenzoic acid) and 4-ampyrone can be catalyzed by peroxidase and form pink compound. Creatinine content can be calculated indirectly by measuring the OD value at 515 nm.

Assay Performance Time

30 min

Sample Type

Serum; plasma; urine

Detection Range

20.45-400 μmol/L

Sensitivity

3.8 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://www.sciencedirect.com/science/article/pii/S0142961221003434

Citation 02

https://www.sciencedirect.com/science/article/pii/S1742706121007108

Citation 03

https://www.sciencedirect.com/science/article/pii/S1742706120307509

Citation 04

https://pubs.rsc.org/en/content/articlelanding/2023/bm/d2bm01836e

Citation 05

https://www.hindawi.com/journals/omcl/2022/1763922/

Citation 06

https://www.mdpi.com/2124124

Citation 07

https://link.springer.com/article/10.1007/s10565-023-09790-0

Citation 08

https://www.mdpi.com/article/10.3390/ijms232315400

Citation 09

https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.7608

Citation 10

https://europepmc.org/article/med/35392552?javascript_support=no

Citation 11

https://www.sciencedirect.com/science/article/pii/S0378874123001113

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164873/

Inter CV

3.7%

Intra CV

1.4%

Detection Instrument

Microplate reader

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

RC222449L3 10 µg

Thyroid Peroxidase (TPO) (NM_175719) Human Tagged Lenti ORF Clone

Ask

View Details

LS114876-100ug 100 µg

ORP1 (OSBPL1A) (NM_080597) Human Over-expression Lysate

Ask

View Details

36-3207-100 100 µg

Anti-Spectrin beta III (SPTBN2) Monoclonal Antibody (Clone: SPTBN2/3142R)

Ask

View Details

TA502005 100 µL

XLF (NHEJ1) Mouse Monoclonal Antibody [Clone ID: OTI3E5]

Ask

View Details

MAB868-SP 25 µg

Human APAF-1 MAb (Clone 94408)

Ask

View Details