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Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit

Product Specifications

Target

ATP Synthase

Type

Colorimetric method

Field of Research

Mitochondria Function; cuproptosis

Assay Principle

Mitochondrial complex V is also known as F0F1-ATP synthase. ATP is hydrolyzed by F0F1-ATP synthase to produce ADP, and ADP converts NADH into oxidized coenzyme I (NAD) after enzyme conversion reaction. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 340 nm.

Assay Performance Time

15 min

Sample Type

Animal tissue; cell

Detection Range

12.13-74.21 U/L

Sensitivity

12.13 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

6.5%

Intra CV

5.6%

Detection Instrument

Microplate reader

Available Sizes

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