Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit
Product Specifications
Target
ATP Synthase
Type
Colorimetric method
Field of Research
Mitochondria Function; cuproptosis
Assay Principle
Mitochondrial complex V is also known as F0F1-ATP synthase. ATP is hydrolyzed by F0F1-ATP synthase to produce ADP, and ADP converts NADH into oxidized coenzyme I (NAD) after enzyme conversion reaction. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 340 nm.
Assay Performance Time
15 min
Sample Type
Animal tissue; cell
Detection Range
12.13-74.21 U/L
Sensitivity
12.13 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
6.5%
Intra CV
5.6%
Detection Instrument
Microplate reader
Available Sizes
Documents
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