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Mitochondrial Complex IV (Cytochrome C Oxidase) Activity Assay Kit

Product Specifications

Type

Colorimetric method

Field of Research

Mitochondria Function; cuproptosis

Assay Principle

Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm.

Assay Performance Time

10 min

Sample Type

Animal tissue

Detection Range

3.0–88.6 U/L

Sensitivity

3.0 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

9.5%

Intra CV

5%

Detection Instrument

Microplate reader

Available Sizes

Frequently Asked Questions

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