Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit
Product Specifications
Type
Colorimetric method
Field of Research
Mitochondria Function; cuproptosis
Assay Principle
Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The specific inhibitor rotenone can inhibit the enzyme, and the total enzyme activity minus the enzyme activity after inhibitor inhibition can reflect its specific enzyme activity.
Assay Performance Time
15 min
Sample Type
Animal tissue
Detection Range
15.58-224.2 U/L
Sensitivity
15.58 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
8.4%
Intra CV
0.8%
Detection Instrument
Microplate reader
Available Sizes
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