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Reactive Oxygen Species (ROS) Fluorometric Assay Kit

Product Specifications

Target

ROS; DCFH-DA; DCFH; DCF

Type

Fluorometric method

Field of Research

Oxidative Stress; Ferroptosis; Metabolic Diseases

Assay Principle

DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin) . In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.

Assay Performance Time

60 min

Sample Type

Fresh tissue; cultured cells

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Citation 01

https://www.sciencedirect.com/science/article/pii/S2211383522004798

Citation 02

https://www.sciencedirect.com/science/article/pii/S0043135420312884

Citation 03

https://www.sciencedirect.com/science/article/pii/S0891584922009455

Citation 04

https://www.mdpi.com/1983336

Citation 05

https://onlinelibrary.wiley.com/doi/abs/10.1111/cns.14085

Citation 06

https://journals.asm.org/doi/abs/10.1128/Spectrum.00074-21

Citation 07

https://www.sciencedirect.com/science/article/pii/S0147651322009770

Citation 08

https://www.ahajournals.org/doi/abs/10.1161/hypertensionaha.118.11035

Citation 09

https://www.tandfonline.com/doi/abs/10.1080/10717544.2022.2147280

Citation 10

https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/biof.1824

Citation 11

https://journals.sagepub.com/doi/abs/10.1177/0271678X211029060

Citation 12

https://static.frontiersin.org/articles/10.3389/fmolb.2022.816510/full

Detection Instrument

Fluorescence Microplate reader; Fluorescence Microscope; Flow Cytometry

Documents

Product Datasheet

Click to open

Frequently Asked Questions

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