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Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)

Selected Variant

E-BC-K125-M-01

Size: 48 T (24 samples)

Product Specifications

Target

ChAT

Type

Colorimetric method

Field of Research

Amino Acids and Proteins

Assay Principle

Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA), CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.

Assay Performance Time

65 min

Sample Type

Animal tissue

Detection Range

1.21-40 U/g fresh we

Sensitivity

1.21 U/g fresh weigh

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Citation 01

https://www.mdpi.com/2050368

Inter CV

9.4%

Intra CV

5.1%

Detection Instrument

Microplate reader

Available Sizes

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