H+K+-ATPase Activity Assay Kit
Product Specifications
Type
Colorimetric method
Field of Research
Oxidative stress
Assay Principle
ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.
Assay Performance Time
60 min
Sample Type
Animal tissue; cells
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Citation 01
https://www.sciencedirect.com/science/article/pii/S0378517321011091
Citation 02
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709249/
Citation 03
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9429807/
Citation 04
https://europepmc.org/articles/pmc9424734/bin/table5.docx?javascript_support=no
Citation 05
https://www.mdpi.com/1420-3049/27/16/5065
Citation 06
https://www.mdpi.com/1420-3049/27/18/5919
Citation 07
https://bmcpharmacoltoxicol.biomedcentral.com/articles/10.1186/s40360-022-00596-0
Inter CV
9.8%
Intra CV
4.4%
Detection Instrument
Spectrophotometer
Available Sizes
Frequently Asked Questions
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