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Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)

Product Specifications

Target

TC

Type

Colorimetric method

Field of Research

Metabolic Diseases; Glycolysis and Lipid Metabolism

Assay Principle

Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of 4-aminoamylpyridine and phenol, hydrogen peroxide catalyze peroxidase to form red quinone compounds of benzoquinone imine phenizone.The color depth of the generated quinone is directly proportional to the cholesterol content. The absorbance values of the standard tube and the sample tube are measured respectively, and the cholesterol content in the sample can be calculated.

Assay Performance Time

50 min

Sample Type

Serum; plasma; hydrothorax; animal tissue

Detection Range

0.09-25.85 mmol/L

Sensitivity

0.09 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13332

Citation 02

https://journals.asm.org/doi/abs/10.1128/mBio.01155-21

Citation 03

https://www.sciencedirect.com/science/article/pii/S1043661819308473

Citation 04

https://www.sciencedirect.com/science/article/pii/S0223523421002373

Citation 05

https://europepmc.org/article/pmc/pmc8689138?javascript_support=no

Citation 06

https://link.springer.com/article/10.1007/s10565-022-09768-4

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279576/

Citation 08

https://pubs.rsc.org/en/content/articlelanding/2023/fo/d2fo03509j

Citation 09

https://www.sciencedirect.com/science/article/pii/S2212429222005399

Citation 10

https://www.sciencedirect.com/science/article/pii/S0378874122009321

Citation 11

https://www.mdpi.com/1861292

Citation 12

https://www.sciencedirect.com/science/article/pii/S037842742100182X

Inter CV

2.8%

Intra CV

1.1%

Detection Instrument

Spectrophotometer

Available Sizes

Curated Selection

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