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Total Glutathione (T-GSH) /Oxidized Glutathione (GSSG) Colorimetric Assay Kit

Product Specifications

Target

T-GSH; GSSG

Type

Colorimetric method

Field of Research

Oxidative stress; cuproptosis; Ferroptosis

Assay Principle

GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.

Assay Performance Time

100 min

Sample Type

Serum; plasma; animal tissue; red blood cells; cultured cells

Detection Range

0.36-30 μmol/L

Sensitivity

0.36 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Citation 01

https://www.sciencedirect.com/science/article/pii/S1385894722054195

Citation 02

https://www.nature.com/articles/s41419-022-05515-z

Citation 03

https://www.mdpi.com/1905716

Citation 04

https://static.frontiersin.org/articles/10.3389/fmolb.2022.816510/full

Citation 05

https://www.sciencedirect.com/science/article/pii/S0891584920311849

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202200341

Citation 07

https://link.springer.com/article/10.1186/s12958-022-00949-8

Citation 08

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03577k

Citation 09

https://www.sciencedirect.com/science/article/pii/S156757692201133X

Citation 10

https://www.sciencedirect.com/science/article/pii/S0300908422000177

Citation 11

https://www.sciencedirect.com/science/article/pii/S0306452223000763

Citation 12

https://www.sciencedirect.com/science/article/pii/S1756464620304229

Inter CV

3.9%

Intra CV

0.6%

Detection Instrument

Microplate reader

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