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Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Product Specifications

Target

GSH-PX

Type

Colorimetric method

Field of Research

Oxidative Stress; Plant Physiology Series; Ferroptosis

Assay Principle

Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG) . The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.

Assay Performance Time

70 min

Sample Type

Serum; plasma; cells; cell culture supernatant; tissue

Detection Range

12.65-387 U

Sensitivity

12.65 U

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://www.mdpi.com/article/10.3390/antiox11112264

Citation 02

https://www.mdpi.com/2072-6643/14/15/3179

Citation 03

https://www.sciencedirect.com/science/article/pii/S0753332221008003

Citation 04

https://www.sciencedirect.com/science/article/pii/S0278691522002599

Citation 05

https://www.mdpi.com/2076-3921/9/3/250

Citation 06

https://www.sciencedirect.com/science/article/pii/S0024320521012170

Citation 07

https://www.hindawi.com/journals/omcl/2020/2978340/

Citation 08

https://www.sciencedirect.com/science/article/pii/S1001074222000717

Citation 09

https://www.mdpi.com/2077-0383/7/10/315

Citation 10

https://pubmed.ncbi.nlm.nih.gov/36160397/

Citation 11

https://www.mdpi.com/1332228

Citation 12

https://www.sciencedirect.com/science/article/pii/S0009279720310875

Inter CV

9.3%

Intra CV

4.9%

Detection Instrument

Spectrophotometer

Available Sizes

Documents

Product Datasheet

Click to open

Frequently Asked Questions

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