Acid Phosphatase (ACP) Activity Assay Kit
Product Specifications
Target
ACP
Type
Colorimetric method
Field of Research
Amino Acids and Proteins
Assay Principle
Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.
Assay Performance Time
45 min
Sample Type
Serum; plasma; hydrothorax; urine; cells; cell culture supernatant; animal tissue
Detection Range
1.40-40 U/100 mL
Sensitivity
1.40 U/100 mL
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2~8°C for 12 months with shading light.
Inter CV
9.4%
Intra CV
2.1%
Detection Instrument
Microplate reader
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