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Acid Phosphatase (ACP) Activity Assay Kit

Product Specifications

Target

ACP

Type

Colorimetric method

Field of Research

Amino Acids and Proteins

Assay Principle

Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.

Assay Performance Time

45 min

Sample Type

Serum; plasma; hydrothorax; urine; cells; cell culture supernatant; animal tissue

Detection Range

1.40-40 U/100 mL

Sensitivity

1.40 U/100 mL

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Inter CV

9.4%

Intra CV

2.1%

Detection Instrument

Microplate reader

Available Sizes

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