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L-Lactic Acid (LA) Colorimetric Assay Kit

Product Specifications

Type

Colorimetric method

Field of Research

Glycolysis and Lipid Metabolism

Assay Principle

Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated.

Assay Performance Time

30 min

Sample Type

Serum; plasma; cell culture supernatant; tissue; cells

Detection Range

0.12-7.0 mmol/L

Sensitivity

0.10 mmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://www.sciencedirect.com/science/article/pii/S0092867422015379

Citation 02

https://www.nature.com/articles/s41423-022-00894-x

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8419046/

Citation 04

https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-022-02521-y

Citation 05

https://www.sciencedirect.com/science/article/pii/S0223523423000818

Citation 06

https://www.sciencedirect.com/science/article/pii/S2352304222002331

Citation 07

https://link.springer.com/article/10.1186/s12964-022-00882-8

Citation 08

https://link.springer.com/article/10.1186/s12935-022-02845-y

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207367/

Citation 10

https://www.mdpi.com/1400748

Citation 11

https://www.sciencedirect.com/science/article/pii/S258900422101138X

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408126/

Inter CV

3.5%

Intra CV

1.4%

Detection Instrument

Microplate reader

Available Sizes

Curated Selection

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