Vitamin C (VC) Colorimetric Assay Kit
Product Specifications
Target
VC; Ascorbic acid
Type
Colorimetric method
Field of Research
Oxidative stress; Plant Physiology Series
Assay Principle
The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.
Assay Performance Time
80 min
Sample Type
Serum; plasma; tissue
Detection Range
0.35-20 μg/mL
Sensitivity
0.35 μg/mL
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2~8°C for 12 months with shading light.
Citation 01
https://www.mdpi.com/article/10.3390/nu15040832
Citation 02
https://www.sciencedirect.com/science/article/pii/S0891584920310911
Citation 03
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403741/
Citation 04
https://www.nature.com/articles/s41598-019-52646-z
Citation 05
https://www.sciencedirect.com/science/article/pii/S0024320520303325
Citation 06
https://www.tandfonline.com/doi/abs/10.2147/IJGM.S402206
Citation 07
https://onlinelibrary.wiley.com/doi/abs/10.1002/jez.2603
Inter CV
3.3%
Intra CV
2.3%
Detection Instrument
Spectrophotometer
Available Sizes
Curated Selection
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