CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

Product Specifications

Target

CuZn-SOD; T-SOD

Type

Colorimetric method

Field of Research

Oxidative stress

Assay Principle

Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.

Assay Performance Time

100 min

Sample Type

Serum; plasma; urine; cells; cell culture supernatant; tissue

Detection Range

2.03-155 U/mL

Sensitivity

2.03 U/mL

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.14263

Citation 02

https://www.mdpi.com/2103898

Citation 03

https://www.mdpi.com/1963432

Citation 04

https://www.mdpi.com/article/10.3390/antiox11112264

Citation 05

https://www.mdpi.com/2076-3921/11/4/792

Citation 06

https://www.mdpi.com/article/10.3390/antiox11061122

Citation 07

https://www.mdpi.com/2076-3921/11/4/668

Citation 08

https://www.hindawi.com/journals/omcl/2020/2978340/

Citation 09

https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/lt.26421

Citation 10

https://www.mdpi.com/1861292

Citation 11

https://www.mdpi.com/2311-5637/8/6/267

Citation 12

https://www.sciencedirect.com/science/article/pii/S0753332220300317

Inter CV

7.2%

Intra CV

Detection Instrument

Spectrophotometer

Available Sizes

Documents

Product Datasheet

Click to open

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

RR213419L4V 200 µL

Olr1072 (NM_001000062) Rat Tagged ORF Clone Lentiviral Particle

Ask

View Details

E30AOC637 100 µL

TRI mL1 Antibody

Ask

View Details