CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

Product Specifications

Target

CuZn-SOD; Mn-SOD; T-SOD

Type

Colorimetric method

Field of Research

Oxidative stress

Assay Principle

Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.

Assay Performance Time

70 min

Sample Type

Serum; plasma; urine; cells; cell culture supernatant; tissue

Detection Range

1.35-62 U/mL

Sensitivity

1.35 U/mL

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.14263

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.14263

Citation 03

https://link.springer.com/article/10.1007/s00204-022-03431-x

Citation 04

https://www.mdpi.com/1926090

Citation 05

https://www.hindawi.com/journals/omcl/2022/7655142/

Citation 06

https://www.sciencedirect.com/science/article/pii/S0024320522001382

Citation 07

https://www.hindawi.com/journals/omcl/2020/8365056/

Citation 08

https://www.mdpi.com/1861292

Citation 09

https://www.sciencedirect.com/science/article/pii/S1001074222000717

Citation 10

https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/lt.26421

Citation 11

https://www.mdpi.com/1409316

Citation 12

https://europepmc.org/article/pmc/pmc9500298?javascript_support=no

Inter CV

9.6%

Intra CV

5.1%

Detection Instrument

Microplate reader

Available Sizes

Documents

Product Datasheet

Click to open

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