Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Product Specifications

Target

T-SOD

Type

Colorimetric method

Field of Research

Oxidative stress; Plant Physiology Series; Ferroptosis

Assay Principle

The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.

Assay Performance Time

30 min

Sample Type

Serum; plasma; hydrothorax; ascites; urine; cells; tissue

Detection Range

0.2 -14.4 U/mL

Sensitivity

0.2 U/mL

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Citation 01

https://www.sciencedirect.com/science/article/pii/S2452199X23000154

Citation 02

https://pubs.acs.org/doi/abs/10.1021/acsnano.9b09974

Citation 03

https://www.sciencedirect.com/science/article/pii/S0304389421020707

Citation 04

https://www.sciencedirect.com/science/article/pii/S2090123222001229

Citation 05

https://pubs.acs.org/doi/abs/10.1021/acsami.1c14201

Citation 06

https://cmbl.biomedcentral.com/articles/10.1186/s11658-023-00416-1

Citation 07

https://www.sciencedirect.com/science/article/pii/S004896972105405X

Citation 08

https://www.mdpi.com/1983336

Citation 09

https://www.mdpi.com/1905716

Citation 10

https://www.sciencedirect.com/science/article/pii/S0925400520305839

Citation 11

https://www.mdpi.com/article/10.3390/ijms232315400

Citation 12

https://www.nature.com/articles/s41419-020-02777-3

Inter CV

3.7%

Intra CV

2.9%

Detection Instrument

Microplate reader

Available Sizes

Curated Selection

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