Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

Product Specifications

Target

T-SOD

Type

Colorimetric method

Field of Research

Oxidative stress; Plant Physiology Series

Assay Principle

The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-) . The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.

Assay Performance Time

70 min

Sample Type

Serum; plasma; urine; cells; cell culture supernatant; tissue

Detection Range

2.4-61 U/mL

Sensitivity

2.4 U/mL

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Citation 01

https://www.sciencedirect.com/science/article/pii/S2452199X23000154

Citation 02

https://www.sciencedirect.com/science/article/pii/S0304389421020707

Citation 03

https://cmbl.biomedcentral.com/articles/10.1186/s11658-023-00416-1

Citation 04

https://www.mdpi.com/1970288

Citation 05

https://www.mdpi.com/2076-3921/11/10/1879

Citation 06

https://www.mdpi.com/1905716

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.14263

Citation 08

https://www.hindawi.com/journals/omcl/2022/2232365/

Citation 09

https://www.mdpi.com/article/10.3390/ijms232315400

Citation 10

https://www.nature.com/articles/s41419-020-02777-3

Citation 11

https://www.sciencedirect.com/science/article/pii/S0147651322000240

Citation 12

https://www.mdpi.com/1422-0067/23/21/13531

Inter CV

5.6%

Intra CV

5.5%

Detection Instrument

Microplate reader

Available Sizes

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