Glucose-6-phosphate (G6P) Colorimetric Assay Kit
Product Specifications
Target
G6P
Type
Colorimetric method
Field of Research
Glycolysis and Lipid Metabolism; Disulfidptosis
Assay Principle
Glucose-6-phosphate (G6P) is a molecule generated by phosphorylation of hydroxyl groups on the sixth carbon of glucose under the catalysis of hexokinase. It is a common small molecule of sugar metabolism in cells and participates in biochemical pathways such as glycolysis and pentose phosphate pathway. In the first reaction of glycolysis, glucose is catalyzed by hexokinase to produce glucose-6-phosphate, which is then catalyzed by phosphoglucose isomerase to form fructose-6-phosphate to continue the other steps of glycolysis: In the pentose phosphate pathway, glucose-6-phosphate is the first substrate, and this process is also the main way to generate NADPH. In addition to these two metabolic pathways, glucose-6-phosphate can also be converted into glycogen or starch and stored.
Assay Performance Time
35 min
Sample Type
Serum; plasma; animal tissue
Detection Range
5.6-500 μmol/L
Sensitivity
5.6 μmol /L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
4.3%
Intra CV
2.1%
Detection Instrument
Microplate reader
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