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Choline/Acetylcholine Fluorometric Assay Kit

Product Specifications

Type

Fluorometric method

Field of Research

Glycolysis and Lipid Metabolism

Assay Principle

Choline and acetylcholine play important roles in many biological processes. Choline is an essential nutrient, often grouped with B complex vitamins, that plays a key role in many biological processes. Choline is a precursor to the synthesis of acetylcholine (AchE), an important neurotransmitter in the peripheral and central nervous systems. Choline is measured by enzyme coupling reaction to generate fluorescent products in this kit, and the content of fluorescent substances is proportional to the presence of choline. Acetylcholinesterase was added to the reaction to hydrolyze acetylcholine into choline and acetic acid, and the acetylcholine content was further calculated by measuring the total amount of choline and acetylcholine.

Sample Type

Serum; plasma; animal tissue

Detection Range

0.06-10 μmol/L

Sensitivity

0.06 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

8.2-10%

Intra CV

3.2-5%

Detection Instrument

Fluorescence Microplate reader

Frequently Asked Questions

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