Aspartate Aminotransferase (AST/GOT) Activity Fluorometric Assay Kit
Product Specifications
Target
AST; GOT
Type
Fluorometric method
Field of Research
Liver and Renal Function
Assay Principle
AST can catalyze the substrate to produce pyruvate. Pyruvate is oxidized to produce hydrogen peroxide, which causes the fluorescent probe to produce fluoresce. The activity of AST can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
Assay Performance Time
80 min
Sample Type
Cell; animal tissue; serum (plasma) ; other liquid samples
Detection Range
0.03-1.5 U/L
Sensitivity
0.03 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
7.3%
Intra CV
2.2%
Detection Instrument
Fluorescence microplate reader
Available Sizes
Documents
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